Accession Number : ADD014505

Title :   A Membrane Based Dot Immunoassay and Method of Use.

Descriptive Note : Patent Application, Filed 30 Jan 89,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Oprandy, John J

Report Date : 30 Jan 1989

Pagination or Media Count : 20

Abstract : Antigens or antibodies are detected using a novel membrane based immunoassay. Known antigens or antibodies which will form complexes with antigens/antibodies to be assayed are spot filtered with pressure through a membrane. The membrane, either by itself or attached to a base material as a test strip, is incubated with a test fluid. Consequently, the resulting antibody-antigen complex is incubated directly or after an intermediate anti-antibody incubation with enzyme conjugated immunoglobulin and exposed to substrate which produces a colored insoluble product if the test target is present. Patent applications. (aw)

Descriptors :   *ANTIBODIES, *ANTIGENS, *IMMUNOASSAY, *PATENT APPLICATIONS, ENZYMES, EXPOSURE(GENERAL), FLUIDS, IMMUNOGLOBULINS, MEMBRANES, SUBSTRATES, TARGETS, TEST AND EVALUATION, COLORS, INCUBATION

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE