Accession Number : ADD014651

Title :   Microbiological Assay Using Bioluminescent Organism.

Descriptive Note : Patent, Filed 21 Dec 87, patented 21 Aug 90,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Stiffey, Arthur V

Report Date : 21 Aug 1990

Pagination or Media Count : 5

Abstract : A microbiological assay based on bioluminesce employing the bioluminescent dinoflagellate Pyrocystis lunula. An oil well drilling fluid sample is prepared according to E.P.A. procedures to obtain a suspended particulate phase sample. An aliquot of the sample is added to a growth medium containing Pyrocystis lunula in suspension. The mixture is agitated to subject th Pyrocystis lunula to a shear stress. Light emitted as a result of the shear stress on the Pyrocystic lunula is measure and compared with a control to determine if there is diminution of light produced by the Pyrocystis lunula in the mixture. Diminution of light production is an indication of the presence of a toxic substance in the sample. Patents. (JS)

Descriptors :   *BIOLUMINESCENCE, BIOASSAY, DINOFLAGELLATA, DRILLING, FLUIDS, GROWTH(GENERAL), LIGHT, MICROBIOLOGY, OIL WELLS, PATENTS, PRODUCTION, SHEAR STRESSES, TOXICITY

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE