Accession Number : ADD015144

Title :   Preservation and Restoration of Hemoglobin in Blood Substitutes.

Descriptive Note : Patent, Filed 9 Aug 88, patented 24 Sep 91,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Stratton, Lewis P ; Farmer, Martha C ; Rudolph, Alan S

Report Date : 24 Sep 1991

Pagination or Media Count : 10

Abstract : A hemoglobin-containing blood substitute preparation is protected from oxidation to methemoglobin. The protected preparation comprises between 10mM and 60 mM of a compound selected from the group consisting of glutathione(GSH), nicotinamide adenine dinucleotide (NADH), nicotine adenine dinucleotide phosphate (NADPH) and mixtures of these in the aqueous phase of a hemoglobin-containing fluid. Oxidized hemoglobin in a preparation can be restored by adding between 10 mM and 60 mM of a compound selected from a group consisting of glutathione (GSH), nicotinamide adenine dinucleotide (NADH), nicotine adenine dinucleotide phosphate(NADPH) and mixtures of these to a hemoglobin-containing fluid in which the hemoglobin has converted in whole or part to methemoglobin, and storing the fluid at storage temperatures for blood products above the freezing point of the fluid for a time sufficient to convert methemoglobin to hemoglobin.

Descriptors :   ADENINE, AMIDES, BLOOD, BLOOD SUBSTITUTES, FLUIDS, FREEZING, GLUTATHIONE, HEMOGLOBIN, METHEMOGLOBIN, NICOTINE, NUCLEOTIDES, OXIDATION, PREPARATION, STORAGE, TEMPERATURE

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE