Accession Number : ADD015750

Title :   Membrane Based Dot Immunoassay and Method of Use.

Descriptive Note : Patent, Filed 30 Dec 91, patented 6 Apr 93,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Oprandy, John J

Report Date : 06 Apr 1993

Pagination or Media Count : 8

Abstract : Antigens or antibodies are detected using a novel membrane based immunoassay. Known antigens or antibodies which will form complexes with antigens/antibodies to be assayed are spot filtered with pressure through a membrane. The membrane, either by itself or attached to a base material as a test strip, is incubated with a test fluid. Consequently, the resulting antibody-antigen complex is incubated directly or after in intermediate anti-antibody incubation with enzyme conjugated immunoglobulin and exposed to substrate which produces a colored insoluble product if the test target is present.

Descriptors :   *ANTIBODIES, *ANTIGENS, *ENZYMES, *IMMUNOASSAY, *IMMUNOGLOBULINS, *SUBSTRATES, *LABORATORY TESTS, *BIOCHEMISTRY, *PATENTS, FLUIDS, INCUBATION, PRESSURE, TARGETS, MEMBRANES(BIOLOGY)

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE