Accession Number : ADD015934

Title :   Microassay on a Card.

Descriptive Note : Patent, Filed 7 Sep 90, patented 6 Apr 93,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Kidwell, David A

Report Date : 06 Apr 1993

Pagination or Media Count : 12

Abstract : A microassay card for a includes an upper layer containing wells for receiving a liquid sample. A second layer of the card, beneath the first layer, includes a supporting surface bound to a reactive species. A third layer includes a superabsorbent support impregnated with an indicator. Typically, the indicator is a substrate for an enzyme, such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme upon the substrate to cause a spectral change in the absorbent layer. By selecting the structure of the first and second layers, the card can be formatted for a displacement assay or a competitive assay. The microassay card of the present invention is particularly useful for drug testing.

Descriptors :   *CARDS, *PATENTS, *SAMPLING, DYES, ENZYMES, HYDROGEN, HYDROGEN PEROXIDE, INDICATORS, LAYERS, LIQUIDS, PEROXIDES, PRECURSORS, SUBSTRATES, SURFACES, DRUGS, DRUG ABUSE, MEDICINE

Subject Categories : Medicine and Medical Research
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE