Accession Number : ADD016345

Title :   Light Emission- or Absorbance-Based Binding Assays.

Descriptive Note : Patent, Filed 9 Apr 92, patented 24 May 94,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Kidwell, David A

Report Date : 24 May 1994

Pagination or Media Count : 20

Abstract : A substance having binding sites for at least two molecules may be detected within a sample. A molecule which can be recognized by the substance is labelled such that when at least two of the labelled molecules are bound the binding sites on the substance, the labels on the molecules electronically interact with each other and vary the wavelength dependance of their spectra. This variation in the spectra of the label can be detected. If the sample is suspected of containing the unlabelled form of a molecule, such as biotin or cocaine, a known amount of the above substance, along with a known amount of the corresponding labelled biotin or cocaine is added to the sample. In this instance. the amount of the suspect molecule in the sample is then determined by the extent to which the variation in the spectra of the label has been reduced. Alternatively, the present invention can be used to determine the binding characteristics of the substance within the sample. The method of the present invention is useful in immunoassays or other bioassays as well as in studies of surface interactions.

Descriptors :   *PATENTS, *LIGHT, *ASSAYING, COCAINE, INTERACTIONS, LABELS, MOLECULES, SITES, SPECTRA, SURFACES, VARIATIONS, VITAMIN B COMPLEX, EMISSION, SAMPLING, ELECTRONICS, ATOMS, IMMUNOASSAY

Subject Categories : Medicine and Medical Research
      Electrical and Electronic Equipment
      Organic Chemistry
      Optics

Distribution Statement : APPROVED FOR PUBLIC RELEASE