Accession Number : ADD016492

Title :   An Enzyme-Based Detector for Trace Metals.

Descriptive Note : Patent Application, filed 22 Mar 94,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Deschamps, Jeffrey R ; Ward, Keith B ; Light, William R

Report Date : 22 Mar 1994

Pagination or Media Count : 20

Abstract : Metalloenzymes are used for trace metal analysis of samples. The metal ion or ions that promote enzyme activity are first stripped from the metalloenzyme to form an apoenzyme. Then, this apoenzyme and a substrate for the metalloenzyme are added to a sample suspected of containing the target metal ion or ions. The substrate/enzyme pair is selected to provide a product whose production directly or indirectly changes an observable characteristic of the system to a degree that is proportional to the amount of product produced. Monitoring this observable characteristic provides an indication of the concentration of the target metal ion or ions in the sample. Because this method is fast and requires little instrumentation, it may be used in the field. In a laboratory setting, with more sophisticated means for measuring the concentration of product produced from the substrate, the method of the present invention provides a quick, precise, accurate, sensitive and reliable method for assaying metal ions. The method of the present invention is particularly useful in the detection of trace metal ions.

Descriptors :   *ENZYMES, *METALS, ASSAYING, DETECTION, INSTRUMENTATION, INVENTIONS, IONS, LABORATORIES, MONITORING, PRODUCTION, SUBSTRATES, TARGETS, CHEMICAL ANALYSIS, PATENT APPLICATIONS, SPECTROSCOPY

Subject Categories : Biochemistry
      Metallurgy and Metallography

Distribution Statement : APPROVED FOR PUBLIC RELEASE