Accession Number : ADD017288

Title :   Microassay on a Card.

Descriptive Note : Patent, Filed 26 Aug 92, patented 29 Nov 94,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Kidwell, David A

Report Date : 29 Nov 1994

Pagination or Media Count : 12

Abstract : A microassay card includes an upper layer containing wells for receiving a liquid sample. A second layer of the card, beneath the first layer, includes a supporting surface bound to a reactive species. A third layer includes a superabsorbent support impregnated with an indicator. Typically, the indicator is a substrate for an enzyme, such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme upon the substrate to cause a spectral change in the absorbent layer. By selecting the structure of the first and second layers, the card can be formatted for a displacement assay or a competitive assay. The microassay card of the present invention is particularly useful for drug testing.

Descriptors :   *SAMPLING, *CARDS, *PATENTS, TEST AND EVALUATION, SOURCES, COMPETITION, LAYERS, PRECURSORS, ENZYMES, DISPLACEMENT, REACTIVITIES, SUBSTRATES, REDUCTION, SURFACES, SPECTRA, ABSORBERS(MATERIALS), DYES, DRUGS, ASSAYING, HYDROGEN PEROXIDE

Subject Categories : Medicine and Medical Research
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE