Accession Number : ADD017379

Title :   Selective Attachment of Nucleic Acid Molecules to Patterned Self-Assembled Surfaces.

Descriptive Note : Patent Application, Filed 1 Dec 94,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Chrisey, Linda A ; Dressick, Walter J ; Calvert, Jeffrey M

PDF Url : ADD017379

Report Date : 01 Dec 1994

Pagination or Media Count : 46

Abstract : Patterns of pre-formed hybridizable nucleic acid oligomers are formed upon a substrate. The substrate is coated with molecules, such as aminosilanes, whose reactivity with nucleic acid molecules can be transformed by irradiation. The coated substrate exposed to patterned irradiation then contacted with preformed nucleic acid oligomers. The binding of the preformed nucleic acid oligomers to the coating molecules may be covalent or non-covalent (for example, ionic bonding or hydrogen bonding). If desired, a heterobifunctional crosslinker may be employed, before or after irradiation, with the coating to promote covalent binding of the nucleic acid oligomers to the coating molecules. Also, the irradiation step may be performed with the assistance of a positive-tone or negative-tone photoresist. jg

Descriptors :   *PATENT APPLICATIONS, *MOLECULES, *SURFACES, *NUCLEIC ACIDS, HYDROGEN BONDS, COVALENT BONDS, REACTIVITIES, SUBSTRATES, PHOTORESIST COATINGS, CROSSLINKING(CHEMISTRY), SILANES, PATTERNS, HYBRID SYSTEMS, ATTACHMENT, IRRADIATION, AMINO ACIDS, OLIGOMERS, MATERIAL FORMING

Subject Categories : Organic Chemistry
      Biochemistry
      Inorganic Chemistry
      Polymer Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE