Accession Number : ADD017508

Title :   Rapid Assay for Detection of Endotoxins Cross Reference to Related Application.

Descriptive Note : Patent Application, Filed 14 Apr 95,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Ligler, Francis S ; James, Elizabeth A

PDF Url : ADD017508

Report Date : 14 Apr 1995

Pagination or Media Count : 44

Abstract : Endotoxin is an extremely powerful stimulator of the immune system. The devastating effects of bacterial infections and septicemia are in large part due to endotoxin. Mortality rates, due to septicemia are high, 60% or more. The most effective treatment of endotoxin related problems is early detection in real time with high sensitivity. Additionally, endotoxin is a significant contaminant in food products and pharmaceutical products. Accurate determination of endotoxin concentration is required prior to product distribution. To satisfy the requirements of industrial production, endotoxin assays must be accurate, rapid and cost effective. Levin and Bang observed in the horseshoe crab (Limulus polyphemus) that blood coagulation was a consequence of gram-negative bacterial infections. See Levin, J., Bang, F.B., 19 THROMBOS. DIATH. HAEMORRH., 186-197 (1968), incorporated by reference herein in its entirety and for all purposes. When an extract of the horseshoe crab blood was prepared and tested, a gelation reaction was observed in the presence of endotoxin. Levin and Bang postulated that endotoxin mediated the gelation reaction of horseshoe crab blood. They further postulated that the gelation reaction was initiated enzymatically. The enzyme responsible for initiating the gelation reaction was identified as limulus amebocyte lysate (LAL). pg5. JMD

Descriptors :   *DETECTION, *PATENT APPLICATIONS, *INFECTIOUS DISEASES, *ENDOTOXINS, *BLOOD COAGULATION, REQUIREMENTS, COST EFFECTIVENESS, REAL TIME, INDUSTRIAL PRODUCTION, DISTRIBUTION, ENZYMES, ACCURACY, RESPONSE, CONTAMINATION, DRUGS, SEPTICEMIA, IMMUNITY, MORTALITY RATE, FOOD, DETERMINATION, ASSAYING, BACTERIAL DISEASES, BLOOD, GRAM NEGATIVE BACTERIA, GELATION, HIGH SENSITIVITY, CRABS

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE