Accession Number : ADD017793

Title :   Freeze Dried Platelets.

Descriptive Note : Patent Application, Filed 20 Oct 95,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Spargo, Barry J ; Emler, Richard G ; Groel, Thomas R

PDF Url : ADD017793

Report Date : 20 Oct 1995

Pagination or Media Count : 22

Abstract : A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then frozen at a temperature below the glass transition temperature of the suspension. A vacuum is placed on the frozen suspension to remove most of the water therefrom. Then, the temperature of the platelets is increased to the supercooled temperature while the vacuum is maintained. After being sealed under vacuum, the lyophilized platelets may be reconstituted to form viable, transfusable platelets. The reconstituted platelets have a high aggregation index, retain normal agglutination and degranulation capability, and are able to participate in clot formation.

Descriptors :   *PATENT APPLICATIONS, *BUFFERS(CHEMISTRY), *BLOOD PLATELETS, *LYOPHILIZATION, TEMPERATURE, WATER, POLYMERS, VACUUM, GLASS, FREEZE DRYING, TRANSITION TEMPERATURE, FREEZING, SUSPENSION DEVICES, SUPERCOOLING, COAGULATION, AGGLUTINATION

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE