Accession Number : ADD017935

Title :   Surfactant-Enhanced Light Emission- or Absorbance-Based Binding Assays for Polynucleic Acids.

Descriptive Note : Patent, Filed 26 Jul 94, patented 14 Nov 95,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Kidwell, David A

Report Date : 14 Nov 1995

Pagination or Media Count : 23

Abstract : Thc fluorescence of polycyclic aromatic labels and excimers of these labels, attached to nucleic acids is greatly enhanced by the presence of quaternary ammonium surfactants having at least one long chain (C4 or greater) alkyl group. This enhancement may be advantageously used in Pi Overlapping Rings Systems Contained in a Homogeneous Assay (PORSCHA) and in conventional assays.

Descriptors :   *EMISSION, *LIGHT, *SURFACE ACTIVE SUBSTANCES, *ASSAYING, *NUCLEIC ACIDS, FLUORESCENCE, POLYMERS, CARBON, TRANSMITTANCE, AMMONIUM COMPOUNDS, AROMATIC COMPOUNDS, QUATERNARY COMPOUNDS, EXCIMERS, ALKYL RADICALS, HOMOGENEITY

Subject Categories : Polymer Chemistry
      Inorganic Chemistry
      Organic Chemistry
      Physical Chemistry
      Optics

Distribution Statement : APPROVED FOR PUBLIC RELEASE