Accession Number : ADD017986

Title :   Optical Immunoassay for Microbial Analytes Using Non-Specific Dyes.

Descriptive Note : Patent, Filed 6 Aug 93, patented 5 Mar 96,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Ligier, Frances S ; Shriver-Lake, Lisa C ; Wijesuriya, Dayaweera C

Report Date : 05 Mar 1996

Pagination or Media Count : 15

Abstract : The presently disclosed invention relates to a method of rapid detection and identification of microorganisms including bacteria, viruses, rickettsiae and fungi. The method involves staining all microorganisms or fragments thereof in a sample. The stained sample is introduced onto an optical waveguide coated with a capture molecule specific for the microorganism of interest, and the bound microorganism or fragment thereof is then optically detected. For example, detection of B. anthracis and Salmonella was achieved in times of approximately one minute. The sensitivity of this method is on the order of about 3 cells/ul.

Descriptors :   *OPTICAL PROPERTIES, *DYES, *IMMUNOASSAY, *MICROORGANISMS, *PATENTS, QUICK REACTION, DETECTION, MOLECULES, BACTERIA, IDENTIFICATION, OPTICAL WAVEGUIDES, FUNGI, VIRUSES, SALMONELLA, RICKETTSIA

Subject Categories : Medicine and Medical Research
      Microbiology
      Coatings, Colorants and Finishes

Distribution Statement : APPROVED FOR PUBLIC RELEASE