Accession Number : ADD018402

Title :   In Vitro Amplification/Expansion of CD34+ Stem and Progenitor Cells.

Descriptive Note : Patent, Filed 28 Oct 93, patented 4 Feb 97,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Davis, Thomas A ; Kessler, Steven ; Robinson, Douglas H

Report Date : 04 Feb 1997

Pagination or Media Count : 24

Abstract : The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34+ stem and progenitor cells which can be used in human therapeutics.

Descriptors :   *PRECURSORS, *IN VITRO ANALYSIS, *PATENTS, *HEMATOPOIETIC CELLS, HUMANS, PEPTIDES, THERAPY, EXPANSION, IMMUNOLOGY, CULTURES(BIOLOGY), BONE MARROW, ENDOTHELIUM

Subject Categories : Information Science
      Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE