Accession Number : ADD018497

Title :   Microcapillary-Based Flow-Through Immunosensor and Displacement Immunoassay Using the Same.

Descriptive Note : Patent Application, Filed 28 Apr 97,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Narang, Upvan ; Ligler, Frances S ; Gauger, Paul R

PDF Url : ADD018497

Report Date : 28 Apr 1997

Pagination or Media Count : 41

Abstract : The present invention is related generally to immunosensors, and more specifically to immunosensors for flow-through displacement immunoassays. Immunoassays exist in a variety of formats that utilize the interaction of antibodies with antigens usually including direct binding, competitive and sandwich assay schemes. The continuous flow immunoassay is a unique displacement assay that measures the dissociation of a fluorescently labeled antigen from an antibody bound on a solid support when the antigen flows past the antibody (USP 5,183,740, issued February 2, 1993 to Ligler et al., the entirety of which is incorporated herein by reference for all purposes.) The displacement of the labeled antigen is proportional to the quantity of antigen present in the sample. Sensitivity of the assay is dependent on the dissociation constant of the antibody and the probability of antigen-to-antibody interaction in the flow system.

Descriptors :   *DISPLACEMENT, *ANTIBODIES, *IMMUNOASSAY, *ANTIGENS, *CAPILLARITY, *SANDWICH CONSTRUCTION, COMPETITION, INTERACTIONS, SENSITIVITY, FORMATS, FLOW, DISSOCIATION, ASSAYING

Subject Categories : Medicine and Medical Research
      Structural Engineering and Building Technology
      Fluid Mechanics

Distribution Statement : APPROVED FOR PUBLIC RELEASE