Accession Number : ADP001840

Title :   Dimethyl Sulfoxide Effects on Isolated Fat Cells,


Personal Author(s) : Wieser,Paul B.

Report Date : JUN 1983

Pagination or Media Count : 6

Abstract : The ability of dimethyl sulfoxide to inhibit insulin-stimulated glucose oxidation is not the result of the increased lipolysis observed in the absence of insulin with DMSO. Free fatty acids have been shown to inhibit insulin action on fat cells but DMSO still inhibited insulin-stimulated glucose oxidation under conditions (0.56 M DMSO plus insulin) in which there was no lipolytic action of DMSO. The lipolytic effect of DMSO and its ability to potentiate lipolysis due to agents such as noreipinephrine or glucagon may result from DMSO's potentiation of cyclic AMP accumulation due to these agents. Cyclic AMP accumulation due to DMSO alone was slight, but there was a potentiation of the rise in cyclic AMP due to norepinephrine or norepinephrine plus theophylline. The mechanism by which DMSO increased cyclic AMP accumulation due to catecholamines could result from stimulation of adenylate cyclase activity. However, DMSO did not stimulate adenylate cyclase activity caused by catecholamines in the presence of a phosphodiesterase inhibitor. A much more likely effect predominates in intact cells, the activation of lipolysis by DMSO may be secondary to an elevation of cyclic AMP accumulation resulting from inhibition of cyclic AMP phosphodiesterase.

Descriptors :   *Methyl sulfoxide, Symposia, Fats, Adipose tissue, Cells(Biology), Glucose, Metabolism, Adenosine phosphates, Sulfides, Sulfones, Methyl radicals

Distribution Statement : APPROVED FOR PUBLIC RELEASE