Accession Number : ADP001861

Title :   Comparison of Dimethyl Sulfoxide Levels in Whole Blood and Serum Using an Autosampler-Equipped Gas Chromatograph,


Personal Author(s) : Garretson,Susan E. ; Aitchison,John P.

Report Date : JUN 1983

Pagination or Media Count : 4

Abstract : Statistical analysis of the data indicates that DMSO can be reliably quantitated with good precision in whole blood even at low concentrations. Whole blood levels averaged 14.5% lower than serum levels in patients, a finding similar to the difference between whole blood and serum levels for ethanol. The autosampler greatly decreased the amount of time and effort required for injecting samples onto the gas chromatograph. Modification of the autosampler to provide a 3-second delay upon injection resulted in excellent chromatography. Although assay volume used with whole blood is the same as that used with serum, required sample volume is less. This is important where sample volume is limited or where multiple samples are needed from a small subject or test animal.

Descriptors :   *Methyl sulfoxide, Blood analysis, Blood serum, Determination, Measurement, Sampling, Automatic, Gas chromatography, Chromatographic analysis, Patients

Distribution Statement : APPROVED FOR PUBLIC RELEASE