Accession Number : ADP001862

Title :   Cell-Cycle Related Events in the Dimethyl Sulfoxide-Induced Commitment of Hepatic Tumor Cells to Enhanced Liver Function,

Corporate Author : SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK

Personal Author(s) : Higgins,Paul J.

Report Date : JUN 1983

Pagination or Media Count : 4

Abstract : The present data indicate that DMSO perturbs the proliferative kinetics of mouse hepatoma cells in vitro. This reduced proliferative capacity and concomitant stimulation in albumin accumulation, as a consequence of exposure to DMSO, are associated with an increase in the percentage of 4C, G2-like, hepatocytes in the culture. This expanding 4C compartment may represent a subpopulation of hepatocytes arrested (or prolonged) in G2. Tsukada and Hirai have shown that albumin synthesis is restricted to the mid S to late G2 phase of the hepatoma cell cycle. The marked increase in albumin accumulation, as a function of DMSO concentration, in the mouse hepatoma cells used in the present study is consistent with this finding. DMSO-stimulated hepatic tumor cells, therefore, may provide a useful in vitro system for investigations into the control of gene expression during the hepatocyte cell cycle.

Descriptors :   *Methyl sulfoxide, Symposia, Liver, Neoplasms, Cells(Biology), Mice, In vitro analysis, Albumins, Stimulation(Physiology)

Distribution Statement : APPROVED FOR PUBLIC RELEASE