Accession Number : ADP001875

Title :   The Effect of Dimethyl Sulfoxide on the Membrane Dynamics and the Phospholipid Composition of Two Different Cell Lines,

Corporate Author : CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE VILLEJUIF (FRANCE) INST DE CANCEROLOGIE ET D'IMMUNOGENETIQUE

Personal Author(s) : Tapiero,H. ; Zwingelstein,G. ; Fourcade,A. ; Portoukalian,J.

Report Date : JUN 1983

Pagination or Media Count : 6

Abstract : Studies in a variety of biological systems have led to the general hypothesis that cells regulate their membrane lipid composition to maintain a level of membrane fluidity that is constant for a given cell type in a given physiological state. To examine this hypothesis, the relationship between membrane dynamics and cellular growth of Friend leukemia cells (FLC) and Raji cells were studied by fluorescence polarization (P) using diphenyl hexatriene (DPH). In these cells, the P value changes as a function of the state of the cells. In FLC, maximum and minimum P values are related respectively to growing and resting state, whereas in Raji cells minimum P values were observed in the growing state. When both cell lines were grown in presence of 2% DMSO, further changes in P values were observed in FLC but not in Raji cells. We therefore assumed that the membrane action of DMSO is related to the structure and/or to the composition of the cell membrane. To support this assumption, the lipid analysis was carried out. Among the total lipid extracted, the relative amount of the neutral lipid fraction differs between FLC and Raji, being 15.4 and 4.5%, respectively.

Descriptors :   *Methyl sulfoxide, Symposia, Membranes(Biology), Phospholipids, Fluorescence, Polarization

Distribution Statement : APPROVED FOR PUBLIC RELEASE