Accession Number : ADP005160

Title :   A Direct in vivo Method for Studying the Percutaneous Absorption of Volatile Chemicals,

Corporate Author : NATIONAL INST FOR OCCUPATIONAL SAFETY AND HEALTH CINCINNATI OHIO

Personal Author(s) : Susten,Allan S. ; Dames,B. ; Niemeier,R. W.

Report Date : MAY 1985

Pagination or Media Count : 15

Abstract : Two general methods are commonly used to evaluate the in vivo, percutaneous absorption of chemicals. These are the indirect excretion analysis method and the more direct method. Direct methods provide better estimates of absorption for chemicals which tend to be very slowly eliminated from the body and permit a more accurate indication of absorption during the periods immediately following dermal application of a test substance. The purpose of this paper is to describe a direct method used in the laboratory to study the in vivo percutaneous absorption of volatile materials. Hairless mice were chosen as the animal model for these studies. A direct approach to studying dermal absorption of volatile materials was made possibly by development of a skin-depot designed to capture the portion of test substances which would normally be lost by evaporation. The paper will describe and discuss the following: the skin-depot design and its application; the general experimental procedures; tests performed to determine the utility of the skin-depot; and a comparison of benzene and toluene data obtained using different methods.

Descriptors :   *TOXICOLOGY, *TOXIC AGENTS, *ABSORPTION, *SKIN(ANATOMY), IN VIVO ANALYSIS, INJECTION, NEEDLES, PERMEABILITY, VOLATILITY, BENZENE, RATS, SYMPOSIA

Distribution Statement : APPROVED FOR PUBLIC RELEASE