Accession Number : ADP008367

Title :   Processing Enzyme Specificity is a Consequence of Pro-Hormone Precursor Protein Conformation,

Corporate Author : MEDICAL COLL OF VIRGINIA RICHMOND

Personal Author(s) : Rangaraju, N. S. ; Harris, R. B.

Report Date : 1992

Pagination or Media Count : 2

Abstract : Gonadotropin associated peptide (GAP)-releasing enzyme and atrial granule serine proteinase (AGSP) which are isolated and purified from bovine hypothalamic neurosecretory granules and bovine atrial granules respectively, are likely processing enzymes of pro-gonadotropin-releasing hormone (Pro-Gn RH)/GAP and pro-atrial natriuretic factor (ANF) respectively. It was of interest to determine whether each enzyme would maintain its specificity when confronted with the processing sequence of the alternate enzyme. To this end, several peptides based on the primary sequency of pro-ANF and pro-GnRH/GAP were prepared which incorporated either the processing site sequence for the relevant enzyme for the processing site sequence for the alternate processing enzyme. Hydrolysis of these peptide substrates by both enzymes was then assessed.

Descriptors :   *ENZYMES, *GONADOTROPINS, PEPTIDES, PEPTIDE HYDROLASES, SERINE, PURIFICATION, BOVINES, HYDROLYSIS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE