Accession Number : ADP008372

Title :   Correlation of Conformation with Antibody Affinity for Fibrinogen Gamma/Chain Carboxyl Terminal Peptide Segment,

Corporate Author : HUNTER COLL NEW YORK

Personal Author(s) : Blumenstein, Michael ; Matsueda, Gary R.

Report Date : 1992

Pagination or Media Count : 2

Abstract : The carboxyl terminal amino acids of the 411 residue fibrinogen gamma-chain play a significant role in blood clotting. One of the steps in the polymerization of fibrinogen to fibrin involves the intermolecular gamma-chain crosslinking of the side chains of Gln(398) and Lys(406) a process catalyzed by activated Factor XIII. The terminal portion of fibrinogen (but not fibrin) gamma-chain also stimulates platelet aggregation, this activity being contained within residues 400-411. Because of the physiological significance of the fibrinogen-gamma C-terminal region, antibodies have been prepared against it. We are using NMR to study the interaction of fibrinogen gamma-chain peptides with monoclonal antibody 4A5.

Descriptors :   *FIBRINOGEN, *CARBOXYL RADICALS, *ANTIBODIES, BLOOD COAGULATION, NUCLEAR MAGNETIC RESONANCE, MONOCLONAL ANTIBODIES.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE