Accession Number : ADP008433

Title :   Synthetic Metalloproteins,

Corporate Author : UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES

Personal Author(s) : Tomich, John M. ; Richards, John H.

Report Date : 1992

Pagination or Media Count : 2

Abstract : With the success of chemical syntheses and purification of large polypeptides (less than 50 amino acid residues) attention now focuses on developing protocols to correctly fold these products to generate species with high levels of bioactivity. The art of 'breathing life' into these molecules requires denaturation/renaturation, reduction/oxidation of sulfhydryls and assembly of holoproteins from apo-forms. We outline herein some of the strategies employed to assemble metalloproteins with activities essentially identical to that of their native counterparts.

Descriptors :   *PROTEINS(CONJUGATED), *ENZYMES, AMINO ACIDS, CYSTEINE, SYNTHESIS, METALS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE