Accession Number : ADP008546

Title :   The Inhibition of Fibrin Stimulated t-PA-Induced Plasminogen Activation by the A Chain Fragment 149-157 of Urokinase,

Corporate Author : HARVARD MEDICAL SCHOOL BOSTON MA

Personal Author(s) : Liu, J. ; Song, A. ; Zhu, D. ; Gurewich, V.

Report Date : 1992

Pagination or Media Count : 2

Abstract : Both urokinase (UK) and t-PA are major fibrinolytic enzymes widely used as efficient anti-thrombosis agents. The t-PA induced plasminogen activation can be strongly stimulated by fibrin. It is due to the binding both of t-PA and Glu-plasminogen to the D-domain of fibrin. The fibrin A alpha 149-161 sequence had been identified as a determinant for this stimulation. By contrast, UK (two-chain form) displayed little fibrin stimulation. A similarity of charge and hydrophobic distribution between UK 149-158 and A alpha 149-161 was found with a pattern-recognition program. The present study was undertaken to understand the structure-function relationship of UK 149-158.

Descriptors :   *FIBRIN, *ANTICOAGULANTS, PLASMIN, ENZYMES, THROMBOSIS, ENZYME INHIBITORS, BLOOD PROTEINS.

Subject Categories : Biochemistry
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE