Accession Number : ADP008886

Title :   A Screen for Endotoxin and Staphylococcal Enterotoxin B (SEB) Antibody in Rabbits.

Corporate Author : ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Personal Author(s) : Traub, Karen ; Traub, Kim ; Brimfield, Alan ; Doebler, Jeff

Report Date : 13 MAY 1993

Pagination or Media Count : 4

Abstract : There is a well defined need for an alternate animal model to the non-human primate when studying the toxic actions of SEB. Studies of the effects of SEB using the NZW rabbit as a model are difficult to interpret because rabbits exhibit endotoxin-like symptoms when SEB is administered. In addition, they do not have an emetic response as do primates. Many earlier studies, using both monkeys and rabbits, failed to pre-screen the animals for presence of endotoxin or circulating antibodies to SEB. Prior screening allows choosing animals that are free from the interfering actions of these two variables for studying SEB effects. The approach of this study is two-fold. First is to establish parameters/methods for an ELISA to detect SEB antibodies, and determine SEB titers and endotoxin levels in naive rabbits. Secondly, we will administer SEB and look for changes in levels of these two toxins. Presently, working parameters for the ELISA to measure SEB titers have been established, and SEB titers and endotoxin levels using a microtiter assay have been measured on a small naive rabbit population.

Descriptors :   *ANTIBODIES, *ENDOTOXINS, *STAPHYLOCOCCUS, ADDITION, ANIMALS, APPROACH, EMETICS, HUMANS, MODELS, MONKEYS, PARAMETERS, POPULATION, PRIMATES, RABBITS, RESPONSE, SIGNS AND SYMPTOMS, VARIABLES, TOXIC DISEASES.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE